Then can target and record during swimming with ephys
Here is 208, colored by its phase:
And can target some other cells, 152, and 154:
Top - electrodes are recording from 152 and 154. Bottom, ephys of 152 and 154 shows oscillations during swim.
Here is the contralateral 154, and 153:
(This is much later in the experiment. The ganglion is dying and the VSD signals are getting weaker. The 153 oscillation is typically much much larger.)
Now we can excite 152 and see its functional coupling:
A. 152 (white) and 154 (cyan) are targeted with ephys. 154 is excited with 0.5 nA of current in a 1Hz square wave. 154, CV and 208 show inhibitory (out of phase) responses to 152 excitation. B. The coherence of voltage signals computed at 1Hz frequency. Red means in phase, cyan means out of phase. The colors are mapped onto components and shown on the ganglion in A. C. VSD traces of cells with significant coherence to 152. D. Ephys traces of 152 (red) and 154. (Bridge is over-compensated for 152, so what appears as down is actually up). Inhibitory response of 154 is seen in ephys trace. Dp nerve gets excited from 152 excitation, but no clear behavioral response.
Then we can inhibit the cell and see inhibitory functional coupling:
A. 152 is inhibited. B. Coherence of VSD signals with 152. C. Traces of cells with sig coherence. D. Ephys of 152 and 154, no clear effect on 154 from inhibtion. Not much happens to DP.
Oddly, with this it appears that the contralateral CV follows the inhibition in phase, while the ipsilateral CV follows out of phase. Perhaps 152 is electrically coupled to contra CV, and inhibits both CV.
Next, target 154. I ended up getting both bilateral pairs in this experiment.
I also tried 153, but by the end the SNR of the VSD was so low that I wasn't confident about anything.
Here's the summary of 152 and 154 from this experiment: